Why is complementary base pairing important in?

Complementary base pairing is very important in the conservation of the base sequence of DNA. This is because adenine always pairs up with thymine and guanine always pairs up with cytosine. … Therefore, complementary base pairing has a big role in the conservation of the base sequence of DNA.

What is cDNA and why is it important?

cDNA is often used to clone eukaryotic genes in prokaryotes. When scientists want to express a specific protein in a cell that does not normally express that protein (i.e., heterologous expression), they will transfer the cDNA that codes for the protein to the recipient cell.

What is meant by complementary in DNA?

Complementary DNA (cDNA) is a DNA copy of a messenger RNA (mRNA) molecule produced by reverse transcriptase, a DNA polymerase that can use either DNA or RNA as a template.

What is the significance of complementary bases in protein synthesis?

By virtue of complementary base- pairing, this action creates a new strand of mRNA that is organized in the 5′ to 3′ direction. As the RNA polymerase continues down the strand of DNA, more nucleotides are added to the mRNA, thereby forming a progressively longer chain of nucleotides (Figure 2).

Why is complementary base pairing important in DNA replication quizlet?

Complementary base pairing is important because the hydrogen bonds between the bases hold the two strands of DNA together and because it serves as a way for DNA to replicate.

Why is the term complementary used in DNA replication process?

Figure 9.8 The two strands of DNA are complementary, meaning the sequence of bases in one strand can be used to create the correct sequence of bases in the other strand. Because of the complementarity of the two strands, having one strand means that it is possible to recreate the other strand.

What is complementary DNA cDNA )? Quizlet?

what is complementary DNA (cDNA) a complementary, single-stranded, DNA molecule to another mRNA or DNA; composed by mRNA via reverse transcriptase.

Why are the complementary base pairing rules so important when A cell needs to copy its DNA?

Why are the complementary base pairing rules so important when a cell needs to copy its DNA? The cells need both strands to be accurate because each strand codes for one half of the gene. As long as there is one strand, a copy can be made by following the pairing rules.

Why is it useful for the base pairs of DNA to be held together by hydrogen bonds and noncovalent bonds?

Complementary base pairs form between nucleotides on opposite strands of DNA in a molecule via hydrogen bonding. These bonds help hold the strands together by specific nucleotide pairing, adenine to thymine and guanine to cytosine.

Why can only complementary DNA be used in engineering a plasmid?

a cloning vector that contains a highly active bacterial promoter just upstream of a restriction site where a eukaryotic gene can be inserted in the correct reading frame. , cDNA must be used because cDNA lacks introns, which cannot be spliced out of the mRNA within the bacterial cell.

What are the steps in making complementary DNA cDNA?

Perform cDNA synthesis

Reverse transcription reactions involve three main steps: primer annealing, DNA polymerization, and enzyme deactivation. The temperature and duration of these steps vary by primer choice, target RNA, and reverse transcriptase used.

What are the steps in making complementary DNA cDNA quizlet?

Terms in this set (51)
  1. cDNA synthesis– incubating the mRNAs with reverse transcriptase and other necessary components.
  2. PCR amplification is performed using primers specific to the gene of interest.
  3. Gel electrophoresis reveals amplified DNA products that contained mRNA transcribed forom the gene.

What important understandings about embryonic development resulted from the research into Bicoid?

What important understandings about embryonic development resulted from the research into bicoid? led to the identification of a specific protein required for some of the earliest steps in pattern formation.

Which of the following is used to make complementary DNA cDNA from RNA?

4.22 Reverse Transcription Polymerase Chain Reaction. Reverse transcription polymerase chain reaction (RT-PCR) is used to make a complementary DNA (cDNA) from the extracted mRNA for finding the specific protein-coding gene or for our cell to acquire the protein encoded in the mRNA.

Why are bacteria ideal workhorses for biotechnology?

Why are bacteria ideal workhorses for biotechnology? Bacteria contains plasmids and can divide rapidly, producing a large population of useful manufacturing bacteria. … Most scientists use plasmids as a vector to transform a new gene into a bacterial host.

How enhancers and activators interact with transcription factors to affect gene expression?

Enhancers can be located upstream of a gene, within the coding region of the gene, downstream of a gene, or thousands of nucleotides away. When a DNA -bending protein binds to the enhancer, the shape of the DNA changes, which allows interactions between the activators and transcription factors to occur.

Why was the discovery of Taq polymerase a breakthrough for this process?

Why was the discovery of Taq polymerase a breakthrough for this process? It can withstand high temperatures, thus, it can be used in PCR quite well.

How does DNA methylation happen?

DNA methylation is an epigenetic mechanism that occurs by the addition of a methyl (CH3) group to DNA, thereby often modifying the function of the genes and affecting gene expression.