What is the difference between thin and thick film?

Thin film has a thickness in the order of 0.1 um (micrometer) or smaller, while thick film is thousands times thicker. However, the main difference is the method used to apply the resistive film onto the substrate. Thin film resistors have a metallic film that is vacuum deposited on an insulating substrate.

Why is it important to make a thin smear?

Why is it important to make the smear thin? thin smear, because the thickness will determine whether or not you can visualize individual cell morphology(form), their arrangements, or details regarding gram reaction or internal structure. … so that you can view individual cells by themselves rather than in a cluster.

What is the disadvantage of a thick smear over a thin smear when staining?

the disadvantages of: 1. thick blood film cannot identify the e.g. Plasmodium species because of dehemoglobinized of RBCs in thick smear. … thin blood film it depend of the thickness of thin smear of the blood film the quality of the smear it depend on the morphology and size of erythrocytes.

Is thick smear more sensitive than thin smear?

Thick smears are 20 times more sensitive than thin smears, but speciation may be more difficult. The parasitemia can be calculated based on the number of infected RBCs. This is a quantitative test.

Why are thick or dense smears less likely to provide a good smear preparation for microscopic evaluation?

Why are thick or dense smears less likely to provide a good smear preparation for microscopic evaluation? It will diminish the amount of light that can pass through making it difficult to visualize the morphology of single cells under the microscope. Some times the stain can’t penetrate all of the bacteria.

What is the significance of thin and thick blood film in the laboratory?

Thick and thin blood smears will let doctors know the percentage of red blood cells that are infected (parasite density) and what type of parasites are present.

What is the advantage of a thick smear over a thin smear quizlet?

Thick smears consist of a thick layer of dehemoglobinized (lysed) red blood cells (RBCs). The blood elements (including parasites, if any) are more concentrated (app. 30×) than in an equal area of a thin smear. Thus, thick smears allow a more efficient detection of parasites (increased sensitivity).

What are the advantages and disadvantages for detecting malaria parasites in thin and thick blood films?

Thick blood film

This is suitable for the rapid detection of malaria parasites particularly when they are few. Advantages: More sensitive by 30 times than thin films because: the blood is concentrated allowing a greater volume of blood to be examined and.

How is thick and thin smear prepared for malaria?

What are the advantages of Kato Katz technique?

The advantages of Kato-Katz are its low cost, short sample preparation time, simple handling and the need of only basic equipment [8,10]. However, the method has a low sensitivity for low STH infection intensities, hookworm eggs disappear after one hour and samples and slides for hookworm cannot be stored [11–13].

What is smear study?

A blood smear is a blood test used to look for abnormalities in blood cells. The three main blood cells that the test focuses on are: red cells, which carry oxygen throughout your body. white cells, which help your body fight infections and other inflammatory diseases.

What’s the advantage of a blood smear compared to a complete blood count?

What’s the advantage of a blood smear compared to a CBC? Blood smears focus on RBC, WBC, platelets, and in some cases can help identify blood parasites. Viruses cannot be identified with this technique due to their small size. Whic structure does Giemsa stain?

What are the factors that affect the thickness and thickness of a blood smear?

The perfect quality smear is influ- enced by three factors: speed, angle and drop size. thinner the smear will be. The slower the slide is moved, the shorter and thicker the slide will be.

What is the importance of preparing a good blood film?

Inadequately prepared smear can present different artifacts and lead to errors in the differential count. Blood films should be made immediately after collection of the blood, because cell morphology deteriorates rapidly after sample collection.

What does a blood smear detect?

A blood smear is a type of blood test. It looks at the appearance, number, and shape of your red and white blood cells and platelets to see whether they are normal. A blood smear can also detect parasites in your blood. It is now more common to have blood analyzed by a computer.

What is the function of platelets?

They form in your bone marrow, a sponge-like tissue in your bones. Platelets play a major role in blood clotting. Normally, when one of your blood vessels is injured, you start to bleed. Your platelets will clot (clump together) to plug the hole in the blood vessel and stop the bleeding.

What are the desirable qualities of a thin blood film?

The film should be 3-4 cm in length. The ideal thickness is such that there is some overlap of R.B.C. throughout most of its length with proper separation and lack of distortion of RBC’s. the end from where the spread had ended is called tail end.

What are two important qualities of a good bacterial smear?

1. Cells that adhere to the slide and does not wash off during staining and washing procedures. 2. No shrinkage of cells.

Why is it important to smear the blood as soon as the drop is placed on the slide?

Common Causes of a Poor Blood Smear • As soon as the drop of blood is placed on the glass slide, there should be no delay in the making of the smear. Any delay, whatsoever, results in abnormal distribution of the white cells with many of the white cells accumulating at the thin edge of the smear.

What is the difference between blood smear and blood film?

Left smear is unstained, right smear is stained with Wright-Giemsa stain. A blood film—or peripheral blood smear—is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically.

Why is it important to produce a good bacterial smear?

The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure.

What characteristic should a perfect bacterial smear have?

An ideal bacterial smear should be thin, semi-transparent, whitish layer or film, circular with diameter 1 cm. (approximately) free from dirt, dust or any contamination.

What happens if a smear is too thick?

Do NOT make your smear suspensions too thick. The dye will not penetrate well, and there will be far too many bacterial cells to see individual shapes and arrangements.